A lot of our volume is concentrated around ports because customers bring products in from overseas.
These areas of reduced volume are concentrated in the posterior and inferior regions of the brain, including the parietal, temporal and occipital lobes, and in the cerebellum.
Network analysis suggests that cargo traffic volumes are concentrated in the catchments of metropolitan regions, where passenger hubs also serve as air cargo bases.
Rich countries dominate the market by volume, but growth was concentrated in emerging markets.
To determine the concentration of nanoparticles in PBS, a known volume of the stock solution was concentrated by centrifugation (Thermo Scientific Sorvall Legend RT + Centrifuge, 3600 g, 7 min), washed with 0.15 M NH4OH twice, rinsed with acetone, and dried at 20 °C.
Collected samples were homogenized in 10 mM Tris-HCl, pH 8 with ice-cold Dounce tissue grinder (Wheaton, MillVille, NJ) and centrifuged twice at 12,000×g for 10 min. Supernatant was concentrated to appropriate volume with vacuum concentrator, separated with 15, 18 and 20% native PAGE and subsequently stained with Coomassie Brilliant Blue R-250 (CBB) (Bio-Rad, Hercules, CA).
After adding of 0.5 volume water the aqueous phase was concentrated on a rotary evaporator prior to freeze drying, yielding what is further referred to as crude extract.
The protein solution was concentrated to a volume of 300 μL using a Vivascience spin concentrator and applied to a Superdex 75 column (Amersham Biosciences).
The sample was concentrated to the stop volume of the concentrator, approximately 50 60 μL.
