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We proposed that there are changes in the stiff structure of the cortical cytoskeleton (with F-actin mainly contributing in its formation), so we decided to determine its content using TRITC-phalloidin, for which the intensity of fluorescence within the cell volume was assessed using confocal microscopy.
To investigate the possibility that cells transplanted at 9dpi affected the ongoing evolution of the injury epicenter, estimated lesion volume was assessed using unbiased stereology.
The tumor volume was assessed using microcalipers every 3 or 4 days after the inoculation of LM8 cells according to the formula: tumor volume (mm3) = S2×L/2, where L and S indicate the size in millimeters of the longest and shortest part, respectively.
Change in brain and lesion volume was assessed using a one-sample t-test.
Bladder volume was assessed using abdominal ultrasound and uroflowmetry was performed.
Spleen volume was assessed using MRI at a central imaging laboratory.
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Within-group changes of GM volume were assessed using a one sample t test.
The effects of treatments on tumour volume were assessed using mixed models analysing the tumour volume at D4, 8, 11, 15, and 18.
In univariate analyses the associations of these two continuous variables with brain volume were assessed using linear and quadratic effects, as well as cubic splines.
The correlation between postprandial changes from baseline in the fullness VAS scores and changes from baseline in total gastric volumes was assessed using Pearson's correlation r.
The statistical significance of differences between genotypes of internal capsule volume measurements was assessed using a one-way ANOVA (SPSS 11.5 software, SPSS Inc., Chicago, IL), with statistical significance considered at P ≤ 0.05.
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