Exact(2)
An early study performed in Ghana reported a lower sensitivity (50%), 65 possibly because of a difference in strain type (most other studies were performed in Asia), an assay cutoff that was set too high, or insufficient blood volume spotted onto filter paper.
Forty seven human blood-fed, 49 chicken blood-fed and 58 wild-caught blood-engorged sand flies were homogenised individually in 50 μL of 0.15 M saline using a cordless motorized pestle and the whole volume spotted onto a Whatman® FTA card.
Similar(58)
In this mode, astrocytes were identified by their EGFP fluorescence in the green channel and a spherical 3D volume (spot) of 6 mm diameter was assigned to the soma of each EGFP-positive cell astrocytes.
From each strain, 20 µl volumes were spotted onto CBM agar and incubated for 48 h.
Based on average spot volume ratio, spots whose relative expression is changed at least 1.5 fold (increase or decrease) between benign and tumors at 95% confidence level (t-test; p<0.05) were considered to be significant.
The decrease of spot volume in spots containing albumin fragments could be also explained by higher proteasome activity in MDS [ 19].
In order to minimize the effect of any experimental factor on a spot volume, each spot volume was normalized to the total optical density in each gel image [ 52].
Based on average spot volume ratio, spots whose relative expression levels were changed at least 2-fold (increase or decrease) among the compared groups were considered to be significant.
A noncontact robotic printer was also employed to prepare E. coli arrays with several nanoliter-volume spots on chemically modified glass [ 50].
The spotted volume of this oligonucleotide solution was 200 pl, producing a spot diameter of approximately 220 µm.
The entire volume was then spotted onto a silica gel plate (Analtech).
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