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The plastic adjustable volume pipette (Eppendorf Research®pro, Germany) was used.
The electrolyte (5 µL) was dropped on the electrodes using a fixed volume pipette, and the contact angles were measured just after dropping.
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To validate the used volumetric algorithms for compatibility with our MRI-system we examined 1000 mm3 water of defined volume (calibrated pipette) in a standardized 1.5 ml Eppendorf tube as an MRI phantom.
The casein layer was removed by BMP solution in 10 mM Tris HCl pH 7.5 (without any marine salt addition) or by Commercial Protease, as powder dissolved in 10 mM Tris HCl pH 7.5; both gelled in 5% Klucel, (Idrossi-propilcellulose) and stratified on the specimen surface by plastic tips of volume variable pipettes.
The robot was programmed to aspirate the whole sample volume into the pipette tip followed by 2 μL of air, and then to deliver the sample to the inlet side of the microchip.
The volume of the pipettes was adjusted to 4 ml, through which the stimulus airstream was directed during the application.
After the entire volume was injected, pipettes were held at the same position for 10 to 15 min and were then carefully and slowly removed from the tissue, in order to avoid backflow of the viral suspension and unwanted contamination in the cerebellar cortex along the pipette tracts.
Before using graduated pipettes check the volume scale and note: Does the pipette empty from full volume to zero or from zero to full volume?
Having noted that dynamicity also, the study applied (to both samples) similar final volume of 50 and pipette dilution factor of 70 at double stranded DNA molecules of 50 μg/ml constant to 1.0 (Oxford Gene Technology 2011); this uniformity challenges component dilution factor.
Pipette required volume of DNA into all sample wells a. TAKE CARE - this pipetting step must be done with great care and precision 35.
A total of 100 μl medium was removed from each well containing 4-day spheroids, and the remaining medium including the spheroids transferred into the prepared 'migration' plate using a multichannel pipette (final volume 300 μl).
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