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Worm samples were prepared by boiling 1 volume of worm pellet in 1 volume of 2× loading buffer for 5 min. Samples were run on a 10% SDS-PAGE gel and then transferred to PVDF transfer membrane (Immobilon).
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This implies that the volume of worm-like particles is almost unchanged after oil sorption.
The volume of worm-like particles before sorption was estimated by analyzing the area occupied by worm-like particles in slices cut from the EG block formed using paraffin.
For 2 volumes of worms washed with M9 buffer, 1 volume of the bleach solution was added.
Since it is easier to generate larger volumes of worms using liquid CeMM than on a NGM plate it is possible to get larger numbers of animals surviving freezing.
In a footnote to the same pages, Wiggins gives a list of other examples of terms which permit individuation but not counting: "wave, volume of fluid, worm, garden, crystal, piece of string, word-token, machine".
The volume of the worm pellet was determined by eye using direct comparisons with known liquid volumes in identical tubes.
Chicago Daily Tribune, January 14 , 1882 page 9, a review of Darwin's volume on worms.
26, page 190 192, a book review of Darwin's volume on worms.
In 10 mm tubing, a starting volume of 1 ml of worms in liquid CeMM completely evaporated in seven days when sealed in a 100 mm petri dish or in six days when sealed in a Kennedy Space Center fixation tube (KFT [ 10]).
The volume of oils sorbed inside worm-like particles does not vary as much as that sorbed among them.
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