Sentence examples for volume of wash from inspiring English sources

The final pellet was suspended in a minimal volume of wash solution.

Wash matrix 4 times with one volume of wash buffer by centrifugation for 5 min at 4,000 rpm.

An equivalent volume of wash buffer was added and cells were triturated at least 50 times with a p1000 pipette.

The clear lysate was loaded into the Ni-NTA column, and washed with optimized volume of wash buffer containing 50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole.

It was concluded that despite the large volume of wash water used, the application of continuous vacuum during washing did not permit sufficient circulation of the particles in the rinse water.

Supernatant was carefully decanted, and the protein-bound resin was resuspended with an equal volume of lysis buffer and allowed to incubate at 4 °C on a rotator for an additional 15 min. The Ni resin was pelleted again, supernatant was decanted (wash 1), and an equal volume of wash buffer (lysis buffer containing 500 mM NaCl and 30 mM imidazole) was added.

In order to improve lignocellulosic LA production from both cellulose and hemicellulose hydrolysates and reduce the volume of washing water, we compared LA fermentation efficiency using NaOH-pretreated and ammonium-hydrogen peroxide NH3-H2O2 -pretreatedd NH3-H2O2 -pretreated straiNH3-H2O2 -pretreated.

Also, the stringency of selection was increased by (i) increasing the volume of washing buffer (ii) increasing washing time and (iii) increasing the amount of FBS from 10% (from 4th round) to 20%.

In 4.2 thousand ha of clear-cut territory, using good machinery, there is an average volume of washed away soil of 12.6 m3.

The optimal parameters of MEPS extraction (type of sorbent, volume of sample, composition, and volume of washing and elution solutions) for these drugs in spiked samples were defined.

After the cell lysate was incubated with beads and washed with HEPES buffer (25 mM HEPES/KOH, 100 mM KCl, pH 7.4) three times, DTT was added to a final concentration of 2 mM, and nutation was continued at 4 °C for another 30 min. The beads were then washed eight times with an excess volume of washing buffer to remove DTT.