Sentence examples for volume of viral from inspiring English sources

The volume of viral concentrate required for this volume was calculated and this mixed with the requisite volume of complete neurobasal medium.

Two-fold serially diluted purified IgG1 or IgG1 containing supernatant was mixed with an equal volume of viral inoculum, followed by 2 hour incubation at 37°C.

Summary of samples from 33 patients with diverse viral load from which all four antigens were amplified is given on Table 3. Whenever possible, a greater volume of viral plasma was used for HIV RNA extraction to achieve higher yields of viral RNA.

Mice in the control group were given the same volume of viral preservation solution (10 mmol/L Tris HCl pH 8.0, 2 mmol/L MgCl2, 4% sucrose).

Semi-confluent monolayers were washed twice with phosphate buffered saline (PBS) and inoculated with a 100 μl volume of viral suspension.

A 0.5 1.0 μl volume of viral particles was injected over the course of 15 30 min. Mice were surgically prepared for behavioral experiments (Boyden and Raymond, 2003) 6 8 weeks after the virus injection.

Simultaneous overexpression of EndoG-FLAG and Bcl-xL was achieved by adding equal volumes of viral stocks for each gene at 6 hours in vitro, washing and replacing with the required medium 24 hours later.

We performed these titering infections with various volumes of viral supernatant such that the titer could be computed from an infection with between 0.5% and 10% of cells green.

Cells were given equal volumes of viral supernatants containing retroviruses carrying each of four pMX expression vectors for POU5F1, Sox2, Klf4, MYC genes on day 0 and day 1.

Three animals were inoculated with an identical volume of the viral stock after heat inactivation (56°C for 30 min) and euthanized four, sixteen or twenty-four hours post inoculation (M-H4.1, M-H16.1 and M-H24.1 respectively).

Transduction was carried out by plating 10 K562 cells in 9.5-cm dishes with 45% RPMI and 45% I-MDM (Iscove's Modified Dulbecco's Medium, CAMBREX Biowhittaker Europe), 10% FBS, 2 mM l-glutamine (CAMBREX Biowhittaker Europe, Milan, Italy), 100 U/ml penicillin, and 100 mg/ml streptomycin in humified atmosphere of 5% CO2/air and adding the decided volume of the viral supernatant.