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Half volume of the medium was changed every 2 3 days.
Half volume of the medium was changed every 2 3 days and neurons were differentiated up to 3 months.
The design showed that four factors including peanut oil, sucrose, ammonium sulfate and volume of the medium exhibited significant affect on lipase production by P. aeruginosa strain.
Even if the volume of the medium were increased, the yields of galactosylated lactoside were almost similar to those obtained from 10.5 ml.
The developed technique allows to obtain both the integral dependences and time frequencies distributions of the fluids over the volume of the medium under investigation.
A 20-mL volume of the medium was poured into a Petri dish (diameter, 90 mm) on a horizontally leveled surface.
Similar(37)
Bacteria cultured in a chemostat were infected with bacteriophages after exchange of five volumes of the medium.
As the volumes of the medium and rinsing fluids were known, the total amounts of SLPI released apically and baso-laterally could be compared.
The same volume of the appropriate medium was added to replace the medium removed.
Culture medium was changed after 48 hours by removing half of the volume of the culture medium and replacing it with fresh medium.
Cells were first grown overnight in PIB medium, washed twice with one volume of 1x M9, and resuspended in an equal volume of the same medium.
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CEO of Professional Science Editing for Scientists @ prosciediting.com