Sentence examples for volume of substrate from inspiring English sources

This paper presents the development of a convection diffusion-reaction model to simulate the behavior of a flow injeconvection diffusion-reactionr with respect to its design and operational paramodels, such as flow rato, fluidic channel dimensimulatelow cell geomethe and volume of subehaviorand enzyme injected.

A 200 μl volume of substrate solution (tetramethylbenzidine) was added into each well, and the solutions were incubated for 30 min at room temperature on the bench-top.

After incubating for one hour at room temperature and washing repeatedly, a 100 µL volume of substrate solution (1-Step Ultra TMB-ELISA; Lot. 34028, Pierce®) was added to each well.

Briefly, 60 µl of supernatant was added in duplicates into a 96-well plate and mixed with an equal volume of substrate solution (7.5 mM p-nitrophenyl-N-acetyl-β-D-glucosaminide dissolved in 80 mM citric acid, pH 4.5).

After incubating for one hour at room temperature and washing repeatedly (at least two cycles of 5 minutes with PBS-Tween 0.05% followed by two cycles of 5 minutes with PBS in an automatic micro-plate washing instrument), a 100 µL volume of substrate solution (1-Step Ultra TMB-ELISA; Lot. 34028, Pierce®) was added to each well.

The total volume of substrate (in ml) in each category was measured by water displacement in graduated cylinders.

The unit cell volume of reduced RebC is 602000 Å, almost twice the volume of substrate-free RebC, because two monomers previously related by a crystallographic 180° rotation shift to be related by a 160° rotation, with consequent alteration of the a, c, and β values of the unit cell (Table 1).

This is explained by a consensus volume of substrates recognized by the protease, defined as the 'substrate envelope', which not only explains specificity but also has significant implications for drug resistance and substrate coevolution (Kolli et al. 2009).

The process of producing basidiome is laborious and time-consuming; it requires large volumes of substrate, space, and qualified labor and these factors hinder research in the laboratory.

Furthermore, as most anaerobic digestion plants use continuously stirred tank reactors, the negative effects of the low pH of the incoming substrate would be minimised by it being immediately mixed with large volumes of substrate at a suitable pH.

To measure luciferase activity, MCF7 cells were lysed with 1 × Reporter Lysis Buffer (no. E397A; Promega, Madison, WI, USA), equal volumes of substrate from the Dual-Luciferase Reporter Assay System (no. E1960; Promega) added and luminescence measured on a Microplate Luminometer LB 96V (EG & G Berthold, Bad Wildbad, Germany).