The volume of stops had been reducing since 2011.
Police have attempted to ease tensions by reducing the volume of stop-and-searches in areas like Tottenham (although during June there were 495 stop-and-searches in Haringey, the fifth highest among the 32 London boroughs).
But "On the Inside" reported in June that the number of shootings and shooting victims has climbed during the Bloomberg years despite the extraordinary volume of stops-and-frisks in the Big Apple.
After the addition of an equal volume of stop buffer (0.05% bromophenol blue and xylene cynol, 20 mM EDTA, 95% formamide), the radiolabeled substrate and product were separated on a standard polyacrylamide denaturing gel and quantified by phosphorimager analysis [21].
Reactions were initiated by adding 12.5 nM 34 bp duplex oligonucleotide containing one-nucleotide gap at position 16, and were incubated at 37°C for 25 min, followed by termination with equal volume of stop dye.
The cleavage reactions in Figures 1, 3 and 4 were started by the addition of enzyme and then incubated at 30°C for the indicated amount of time before being stopped by the addition of one-fifth volume of stop mixture (2.5% SDS, 200 mM EDTA, 10 mg/ml proteinase K) followed by a further 15 min at 30°C to deproteinize the mixture.
The reactions were stopped using an equal volume of stop buffer (2 % SDS, 200 μg/ml PK, 10 mM EDTA).
To preserve the RNA, 0.1 volume of stop solution was added to the collected bacteria and stored at –80°C.
Digestion was stopped by addition of an equal volume of stop-lysis buffer (50 mM Tris HCl, 600 mM NaCl, 100 mM EDTA, 1 % SDS, pH 7.9).
The reaction was allowed to proceed for 4 h, and then an equal volume of stop solution containing 80% (v/v) formamide and 10 mM EDTA was added.
