Sentence examples for volume of staining from inspiring English sources
In brief, tissues were harvested directly into a volume of staining solution sufficient to cover the tissue and placed under a house vacuum for 10 min. Staining was carried out at 37°C in the dark for 48 hr.
This method can be performed without a microscope, but requires a large volume of staining reagents, and the sensitivity is low.
Cells were then counterstained with 0.5 μg/ml PI on ice for 5 min, and an equal volume of staining buffer was added.
The volume of interest was calculated by quantification of the volume of staining referenced to the surface area of basal lamina surveyed (cubic micrometre per square micrometre).
This volume of staining solution remained on the surface through surface tension during a 60-min staining period at room temperature in the dark.
At 4 hr post exposure, 20 µl of the cell suspension were mixed with equal volume of staining solution (100 µg/ml of propidium iodide and 0.5 µg/ml of acridine orange in phosphate-buffered saline).
Remaining GUS stain was analyzed for intensity and area of staining using the 'volume analysis' tool from BioRad Gel-Doc imaging software.
Inter-loci distances were normalized to nuclear diameter: first, the nuclear volume was calculated from the total volume of DAPI stained DNA; a spherical approximation was then used to calculate the diameter of the nucleus from this volume.
We determined the differential effect of staining on brain tissue distortion through preliminary comparison of gelled and non-gelled brain part volumes before and after iodine staining, as determined by μCT.
For one antibody we found a strong correlation between the volume of a prominently stained ~50 kDa band in human serum, which is clearly not irisin, and irisin levels measured with the corresponding ELISA.
The mean volume of the vessels stained with AngioSense 750 was 2.2 times smaller in Ctsb−/− APCΔ468 adenoma (6963±1236 µm3, Fig. 7 d&h), and 4.2 times smaller in the anti-TNFα treated APCΔ468 adenoma (3700±1444 µm3, Fig. 7 f&h), as compared to the polyps in APCΔ468 mice (15501±2144 µm3, Fig. 7 b&h).
