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Amongst the available analytical techniques, UHPLC has gained a considerable attention due to use of Acquity BEH column, which not only increased the separation throughput and efficiency but also reduced the retention time and volume of solvent required during separation [14 16].
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Experimental data collected from the series of liquid samples analysis made with use of in-needle device showed that the effectiveness of the system depends on various parameters such as breakthrough volume and the sorption capacity, effect of sampling flow rate, solvent effect on elution step, required volume of solvent for elution step.
The surface area, pore volume and pore diameter were increased at lower volume of solvent, whereas they decreased by increasing the volume of solvent.
Corresponding control cultures received an equal volume of solvent (DMSO).
Final concentration of solvent did not exceed 0.2% (v/v); control cultures received the same volume of solvent as for treated counterparts.
Control cells were treated with an equal volume of solvent.
The control group received an equal volume of solvent intravenously.
The control group received an equal volume of solvent control.
Higher volume of solvent resulted in uniform mixing of drug and solvent which resulted in higher EE.
For the negative control plants, an equal volume of solvent (DMSO) was added instead of TM.
Control cells were always treated with an equal volume of solvent.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com