The ability to detect multiple biomarkers from the same sample allows for better diagnoses, more efficient testing, and lower volumes of sample required.
The sheer volume of samples required setting priorities among biotic groups for processing, leaving some more difficult groups unsorted.
These were outlined by SooHoo and Walker: (1) a large volume of sample is required, (2) separation can take up to 20 min, (3) multiple separations may be required, and (4) continuous processing is difficult [ 20].
The present methodology employs the application of larger acoustic actuator without compromising the volume of sample fluid required.
A future study including a larger volume of samples is required to further determine the role of promoter methylation in the RFC transcription regulation.
For TEM analysis, required volume of sample was sonicated in acetone (1 %, w/v) and applied over carbon coated copper grid.
These concerns also exist for the process development laboratory, where despite the smaller scale of operations, the volume of experiments (hence cytotoxic samples) required to define a chemical process is often more significant.
However, these methods are time-consuming or require expensive equipment, skilled operators, often require a large volume of sample and cannot be used for real-time measurements.
The small volume of blood required and variety of sample (whole blood, serum or plasma) that can be used for the test as well as a convenient test procedure and easy interpretation of results make the method attractive and user-friendly.
Compared with previously reported methods, the current method requires a small volume of sample and simple sample preparation steps for sensitive determination of NSAID levels using a conventional GC MS system.
