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Volume of sample was decreased 7.9% by pre-setting pressure.
Subsequently, libraries were enriched with eight cycles of PCR using the entire volume of sample as template.
This new setup provides effective and reproducible extractions with low volume of sample solution.
The developed biosensor showed good sensitivity, fast response and reproducibility with a small volume of sample.
Additionally, the volume of sample in each well of the Echo qualified 384-well source plate must be 15 65 μL, resulting in 15 μL of unusable sample.
The development of Luminex multi-analyte profiling (xMAP) technology helps overcome these obstacles by allowing for the simultaneous measurement of multiple analytes from a small volume of sample.
However, the volume of sample buffer must be restricted to 0.25 ml with the small specimens as compared to the volume (2 ml) for the standard skin biopsies.
The present methodology employs the application of larger acoustic actuator without compromising the volume of sample fluid required.
In the third design, a fixed volume of sample (2 μL) was injected while the CL reagents were continuously infused.
Zika virus RNA was isolated from samples and measured by qRT-PCR according to methods described previously20 modified to increase the initial volume of sample tested from 140 to 200 µl (when available) to increase sensitivity.
The column is loaded with a defined volume of SP Sephadex C-25 cation-exchange resin beads and subsequently exposed to a metered volume of sample solution.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com