We used a synovial fluid volume >0.3 ml, a displacement volume of the round ligament of the femoral head >0.8 ml, and a perifoveal articular cartilage lesion of fibrillation or ulceration as incontrovertible evidence of the presence of hip OA.
Use the shorthand formula for finding the volume of a round pool.
At least until you turn it on or off and the bleep is emitted at the volume of an artillery round.
The final 25 μl volume of the 2nd round reaction mix for Chlamydia spp contained 10 pmol and 25 pmol of the inner primers CHL2C and CHL2D respectively, 10 mM Tris-HCl pH 9.0, 3.5 mM MgCl2, 50 mM KCl, 0.2 mM for each dNTP, 0.1% Triton X-100 and 0.625 units of Taq enzyme.
Today, although it is unlikely to feature in the essential criteria, given the volume of applicants, the first round of shortlisted candidates is often mostly composed of those with a masters.
The final 25 μl volume of the the 1st round reaction mix for Chlamydia spp. nPCR contained 5 pmol and 25 pmol of the outer primers CHL2A and CHL2B respectively, 10 mM Tris-HCl pH 9.0, 3.5 mM MgCl2, 50 mM KCl, 0.2 mM for each dNTP, 0.1% Triton X-100 and 0.625 units of Taq enzyme.
The final 50 μL PCR volume of the first PCR round contained 5 μL of the DNA extraction, primers EphplgroEL 569)F and EphplgroEL(1193 R, and HotMaster Taq DNA polymerase (5u/μL, Eppendorf) according to the manufacturer's basic protocol (Eppendorf AG, Hamburg, Germany).
Because the average volume of each Golgi section represented approximately 20% of the total stack volume (conservatively assuming 'idealized' stacks made of round cisternae of about 1 micron in diameter), the number of connections detected in each tomogram was multiplied by five to obtain the total number of connections per stack.
For DC T cell cultures, 1×104 DCs and 1×105 T cells were cultured in a volume of 200 µl in round-bottomed 96-well plates (Corning) and incubated at 37°C for 4 days in RPMI 1640 media.
Two × 105 PBMC or SFMC were incubated with 0, 3 and 30 μg/ml of each peptide in the presence of RPMI containing 10% human serum in a final volume of 200 μl in round-bottomed wells for 5 days.
