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The surface runoff, which is nearly one-third of the volume of precipitation, moves from hillside into channels that converge into larger channels.
Karstification of the Keserouane limestone was further intensified by the steep topography of the area and the volume of precipitation over the Lebanon (more than 1200mm).
Briefly, each CSF sample was first mixed with 3-fold volume of precipitation agent 1 and kept on ice for 15 min, and then 3-fold sample volume of precipitation agent 2 was added.
Although the volume of precipitation is unchanged from historic conditions, increasing air temperature can change the form of precipitation (typically from snowfall to rainfall), which is discussed with model results.
The supernatant was collected and stored on ice while the pellet was resuspended in a minimal volume of precipitation buffer (50 mM Tris HCl (pH 7.6), 10 mM NaCl, 2 mM EDTA, 1 mM PMSF, 10 μg/ml aprotinin, 1% Igepal, 5 mM sodium butyrate and DNAse to a final concentration of 5 μg/mL), incubated for 30 minutes at room temperature to degrade genomic DNA, and centrifuged (17 500 × g, 4°C, 1 min).
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The protein supernatant was precipitated using 5× volumes of precipitation solution (ethanol:acetone:acetic acid at 50 50 0.1 volume ratio) at 4°C overnight, followed by centrifugation.
Proteins were precipitated from phenol phase using five volumes of precipitation solution (0.1 M ammonium acetate and 1% β-mercaptoethanol in methanol).
Historically, drywells have predominantly been used as a form of stormwater management in locations that receive high volumes of precipitation; however the use of drywells is increasingly being evaluated as a method to supplement groundwater recharge, especially in areas facing severe drought.
The aqueous phase was then transferred and 3 to 3.5 volumes of precipitation buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA, 1% w/v CTAB) were added.
( P_{i} left( t right) ) denotes the intensity of precipitation in city ( i ) at time ( t ), and ( DR_{i} left( {P_{i} left( t right)} right) ) denotes the loss ratio of traffic volume with given precipitation intensity, which can be deemed as the direct adverse impact of precipitation on city ( i ).
One volume of protein-precipitation solution (1 N HCl and 10% (v/v) trichloroacetic acid in high purity water) was then added to the samples, blank and iron standards and incubated for 1 hour at 95°C.
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