Sentence examples for volume of pm from inspiring English sources

Dissolve the pellet in a minimal volume of PM buffer and dialyze against polymix with 3 dialyze changes.

For cells seeded onto Petri dishes for biochemical studies, a further 250 μL of PM was added, making a total volume of PM of 750 μL per Petri dish.

For coverslip cultures, the volume of PM was made up to a total of 500 μL in the Petri dish, to which 500 μL of DfM + insulin was added, ensuring that the coverslips were firmly pressed down onto the base of the dish and fully submerged in the medium.

With the exception of an incomplete notation for relative types in Volume II, the reader should be able to work out all of the rest of the notation in PM using the explanations above and the definitions in the lists at the end of volume I of PM.

Rev was diluted in gel shift buffer and mixed with an equal volume of <25 <span class="lh">pM P-RNA.

Centrifuge at 4000 g for 20 min (4°C) and dissolve [H]fMet-tRNAfMet in a minimal volume (2 ml) of PM buffer.

Reactions were carried out with 200 μg of protein and 800 μg of ONPG (o-nitrophenyl-β-D-galactopyranoside) as substrate in a final volume of 1 ml of PM-2, and incubated at 37°C for 1 h.

In a reaction volume of 200 μl, 75 pM (∼60,000 cpm) I-hCGRP, various concentrations of unlabelled hCGRP competitor ligand (1 μM to 1 pM) and COS7 membranes expressing the receptor of interest were combined.

The volume (V) of each of the three PMs was estimated using the formula V = πd 2 L4−1 where d = diameter of PM and L = length of PM.

The reaction is completed in total volume of 25 μl with 10 pM primer.

The sequencing reaction was performed by PCR amplification in a final volume of 10 μl using 0.05 pM of plasmid, 4 pmoles of primer, and 1 to 3 μl of Big Dye Terminators premix, according to Applied Biosystems protocol.