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The experimental parameters including accumulation time, pH value of buffer solution, and the volume of modified composite were optimized.
The results showed that the secondary pore volume of modified samples could reach 0.145 cm3/g, 43.9% of the whole pore volume, and the amount and intensity of medium strong acid as well as the thermal stability of modified USY were favorable.
The secondary pore volume of modified USY zeolite increase with the increase of reaction temperature, which is to be expected.
The secondary pore volume of modified USY zeolite increases with the increase of reaction temperature as expected.
As shown in the table, with the increase of the concentration of CA solution, the secondary pore volume of modified USY zeolite increases firstly and then decreases.
It can be seen from Table 1 that the surface area and pore volume of modified meso-SAPO-11 with citric acid at 23 °C did not change obviously.
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The samples were homogenized on ice with a homogenizer in at least 5 volumes of modified RIPA buffer (Tris 50 mM, pH 7.4, NaCl 150 mM, EDTA 1 mM, SDS 0,2%%) supplemented with cocktail inhibitors protease.
The resulting precipitate was re-homogenized in 3 volumes of modified RIPA buffer (50 mM Tris [pH 7.4], 1% NP-40, 0.25% Sodium deoxycholate, 150 mM NaCl, and 1 mM EGTA) and centrifuged at 100,000g for 20 min at 4°C.
For Western blot analysis, each sample was homogenized in 5 volumes of modified RIPA buffer containing 150 mM NaCl, 50 mM Tris HCl (pH 8.0), 1 mM EDTA, 1% IGEPAL, 0.5% sodium deoxycholate, 0.1% SDS and protease/phosphatase inhibitor cocktail (Calbiochem, La Jolla, CA), and centrifuged at 10,000 g for 10 min to remove any insoluble material.
The immunocomplex beads were then harvested by centrifugation at 3,000 rpm and 4°C for 5 min and washed three times with 10 bead volumes of modified RIPA buffer.
The total volume of the modified ICSSWH has two sections.
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