Sentence examples for volume of loading from inspiring English sources

A 0.1* volume of loading buffer (containing 0.3% bromophenol blue, 50% glycerol, 0.3% mercaptoethanol, and 50% [v/v] lysis buffer) was added.

The supernatant was taken out and mixed with the same volume of loading buffer and then was analyzed with SDS-PAGE.

For HIC, two 5-mL HiTrap Octyl FF columns (GE Healthcare) were serially connected and equilibrated with one column volume of loading buffer (10 mL of 200 mM ammonium sulphate in 50 mM Tris-HCl buffer, pH 7.0).

Pellet volume was estimated and re-suspended into twice the volume of Loading Buffer.

The reactions were stopped by the addition of one volume of loading buffer, fractionated on denaturing 5 8% PAGE gels which were then exposed to PhosphorImager screens.

An equal volume of loading dye-urea-TTE was added and products were fractionated by electrophoresis through a 20% denaturing polyacrylamide gel.

AS/RS structures are designed according to their different size, weight, and volume of loads to be handled, and characterized of the warehouse.

The reactions were stopped by the addition of two volumes of loading buffer.

Refolded protein was isolated via affinity chromatography using mannose-Sepharose, with washing in 10 column volumes of loading buffer and eluting in 10 mM Tris, 150 mM NaCl, 2.5 mM EDTA.

After 1 10 dilution in 50 mM sodium phosphate loading buffer pH 7.0, the solution was loaded on a 1 mL HiTrap-HIS column (GE Healthcare) that was previously loaded with 1 mL 0.5 M NiSO4 and washed with 10 column volumes of loading buffer.

Unbound proteins were removed using 10 column volumes of loading buffer followed by 10 column volumes of wash buffer containing optimised imidazole concentrations (data not shown) (50 mM NaH2PO4, 300 mM NaCl, 5 mM imidazole for pQE9, 15 mM for pET15b, pH 7.0).