Sentence examples for volume of library from inspiring English sources

The volume of library to add to an aliquot of Sequence Capture Beads is calculated using the equation (1): (1) μL of library = molecules per bead × 10 million beads library concentration molecules / μL The steps from emulsion formation to biotin streptavidin-enrichment were carried out according to the manufacturer's protocols.

We report a cost-effective and fully scalable, pre-hybridization multiplexed in-solution capture method, amenable to <100 ng of input DNA per person due to removal of sample transfers during library preparation enabled by the constant presence of SPRI beads and resulting in a 50% reduction in the volume of library preparation reagent necessary.

Equal volumes of libraries were diluted in the hybridization buffer, heat denatured and sequenced.

Equal volumes of libraries were pooled and subjected to multiplex sequencing on the MiSeq sequencer (Illumina).

It's hard not to be moved by spaces like his majestic plaza overlooking the Pacific Ocean at the Salk Institute in La Jolla, Calif., or the soaring central volume of his library at Phillips Exeter Academy in New Hampshire.

He seems to know nearly every volume of the library's collection and is eager to pull the crowd-pleasers, like intricate ink sketches suggestive of Jules Verne, or a clown pop-up book.

Because adapter dimers were problematic for one of the pools, all pools were cleaned following the paramagnetic bead-based cleaning procedure in the NEBNext protocol but with 0.8 volumes of AMPure beads to 1 volume of enriched library.

The emPCR titration method, as described in the same manual, was used to determine the optimal volume of amplicon library to use for emPCR amplification, such that 10-12% of the capture beads become enriched.

The total volume of the libraries was reduced using a SpeedVac and amplicons were size-selected at 420 bp ±20 % using the Caliper XT (Perkin Elmer, Waltham, MA, USA).

Some 150 volumes of its library were confiscated and removed to Hereford Cathedral Library.

Libraries were diluted to equal molarity and finally pooled by using equal volumes of each library.