Volume of labeled ionic solution = 250 mL.
The volume of labeled DNA was reduced below 15.7 µl in vacuum centrifuge, and 2.5 µl of yeast tRNA (10 mg/ml, Invitrogen Life Technologies, Carlsbad, USA), 4.3 µl of 20×SSC (1×SSC was 8.8 g/l NaCl and 4.4 g/l trisodiumcitrate) and 0.8 µl of 10% SDS were added.
The culture was replenished with an approximately equal volume of labeled media after sample collection.
Incubate the cells together with the indicated volume of labeled antibody on ice for at least 30 minutes.
After PBTw washing, cichlid fins were prehybridized (50% formamide, 5 × SSC, 1 mg/ml tRNA, 50 μg/ml heparin, 0.1 % Tween-20, 9 mM citric acid, pH 6.0) and hybridized at 69°C overnight in hybridization buffer plus 1/10 volume of labeled probe.
Fine-grained visual categorization has recently received great attention as the volumes of labeled datasets for classification of specific objects, such as cars, bird species, and air-crafts, have been increasing.
Comparison of the volumes of labeled boutons with unlabeled boutons forming synapses on the same or neighboring dendrites showed no significant difference between the two populations.
In order to quantify the titer of unlabeled and labeled hRSV, aliquots of frozen virus were thawed, and equal volumes of labeled and unlabeled virus (from Virus Propagation section) were serially diluted from 10 2 to 10 6 and used to inoculate 24-well plates of 100% confluent Vero cells in quadruplicate.
A 1 2 dilution series of unlabeled PtAu1aLOV was prepared over an appropriate concentration range using a buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl and 10% (w/v) glycerol and were mixed with equivalent volumes of labeled PtAu1aLOV (final concentration 20 nM).
Equal volumes of labeled Cy3 and Cy5 mixes (12.5 μL each) were combined with 25 μL 2X formamide buffer (Vial 7, 3DNA Array350 kit) and the 50 μL hybridization mix added to the gap between the 42°C pre-warmed slides and the pre-placed m-Series lifter-slips.
