Sentence examples for volume of isolation from inspiring English sources

The pellet was resuspended in an appropriate volume of isolation buffer, and centrifuged at 12,000×g for 8 min.

The pellet was resuspended in half of the original volume of isolation buffer and centrifuged in the way mentioned above.

The pellet was resuspended in an appropriate volume of isolation buffer without BSA and the protein concentration was determined according to [19].

The supernatants were collected in separate tubes and the pellets re-suspended in half a volume of isolation buffer each, and homogenized and centrifuged as before.

21 The mitochondrial pellet was washed twice and resuspended in a minimal volume of isolation medium and kept on ice until used.

The suspension was diluted with an equal volume of isolation medium supplemented to 0.2% (wt/vol) with defatted BSA and homogenized in a Potter Elvehjem homogenizer with a loose-fitting Teflon pestle (10 strokes).

The final mitochondrial pellets were re-suspended in minimal volumes of isolation buffer, aliquoted and stored at − 80°C.

Cells were uncoupled from the magnetic beads by incubating at 37°C for 15 min with equal volumes of isolation buffer and DETACHaBEAD CD34 liquid.

Cells were pelleted and washed in cold PBS and resuspended in 10 volumes of isolation buffer (250 mM sucrose, 10 mM HEPES, 1 mM EDTA, pH 7.35).

Cells were scraped and washed twice in PBS before being resuspended in five volumes of isolation buffer (250 m M sucrose, 20 m M HEPES, pH 7.5, 10 m M KCl, 1.5 m M MgCl2, 1 m M EDTA, 1 m M EGTA, 1 m M DTT, 0.2 m M PMSF).

Samples were immediately transferred into an equal volume of RNA isolation buffer and stored frozen at −20 °C until further analysis.