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This immense volume of fresh water dilutes the ocean's saltiness for more than 100 miles (160 km) from shore.
Aliquots of 2 mL were withdrawn periodically and replaced with the same volume of fresh medium.
The release medium was withdrawn for each juncture and replaced with equivalent volume of fresh buffer.
At scheduled time intervals, the release medium was withdrawn and replaced with the equal volume of fresh, filtered medium.
The media was then replaced with an equal volume of fresh DMEM containing BTB extract (25 200 µg/ml).
At the pre-determined time points, the supernatant was collected and replaced with same volume of fresh PBS solution.
At predetermined time intervals, 1,000 µL of PBS was sampled, and replaced with the same volume of fresh PBS.
At chosen time points, 1 mL release medium was withdrawn and replaced with an equal volume of fresh medium.
At predetermined time intervals, the release medium was sampled and replaced by an equal volume of fresh buffer to maintain a constant volume.
At suitable intervals, 3 mL samples were taken from the release medium and an equivalent volume of fresh medium was added.
The supernatant is saved, and mixed with an appropriate volume of fresh SPRI beads.
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CEO of Professional Science Editing for Scientists @ prosciediting.com