Frozen fresh algal cells were extracted in three times the volume of extraction buffer (100 mM Hepes-NaOH (pH 8.0), 10 mM MgCl2, 0.4 mM EDTA, 1 % polyvinypyrrolidone, 100 mM Na-ascorbate, 0.1 % BSA at 0 4 °C) and then stirred for 2 min with a vortex at a maximum speed.
The injection volume of extraction was 5 µL and the autosampler temperature was 4 °C.
volume of extraction, eluent and disperser solvent, pH, adsorbent mass and ultrasonication time has significant influence on methods efficiency.
The effective parameters (factors) of USAEME including volume of extraction solvent (C2H4Cl2), extraction temperature and ultrasonic time were optimized by using a central composite design (CCD).
The buffer pH, the volume of extraction and disperser solvent, and the amount of salt were optimized for full factorial design.
The potentially variables were the volume of extraction and disperser solvents, pH of sample solution, salt effect, temperature, stirring rate and vortex time in the optimization process.
However, the volumetric yield (mass of recovered solute per unit time and per unit volume of extraction bed) of pellets was 34% higher than that of control substrate.
The optimal conditions were: volume of extraction solvent, 113 μL; concentration of the chelating agent, 540 mg L−1; and pH, 6.70.
Several factors influencing the extraction such as the type and volume of extraction solvent, sample pH, extraction time and ionic strength were investigated and optimized.
Factors affecting the extraction process, such as type and volume of extraction and dispersive solvent, extraction time and centrifugation step were investigated thoroughly and optimized utilizing factorial design.
From selected parameters, volume of extraction solvent, volume of dispersive solvent, and salt concentration were optimized by means of experimental design.
