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The final volume of each sample was 100 µl/well.
The volume of each sample was 15 µL.
The mass and bounding volume of each sample were also measured to calculate the resulting relative density.
Sampling was carried out once a week and the volume of each sample 2 L was selected.
The taken volume of each sample is checked gravimetrically according to their initial densities by an AND balance with ± 10−4 g resolution (GR-300).
A 20 μL volume of each sample was injected into a Luna 5 μm HILIC 200 Å 250 × 4.6 mm LC column (Phenomenex, Torrance, CA).
An equal volume of each sample (500 μL) was added to the donor chamber, and 4.5 mL of fresh PBS (pH 6.0) was added to the receptor chamber.
From the volume of each sample and the activity concentration of the stock solution, the activity in each test tube was determined (approximately 5 kBq).
In the histogram shown in Fig. 11, we can see the comparison between the actual volume and the calculated theoretical volume of each sample for the corresponding amount of moles of aluminium used.
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Thus, the design of the reference structure is based on two principles: (1) the volume of each sampling cell is to be as equal as possible; (2) the sampling cells are symmetric along the axis of the monitored region.
An equal volume of 2x sodium dodecyl sulfate (SDS) sample buffer was added, and samples heated to 100°C for 5 min. Equal volumes of each sample were then electrophoresed on a 10% SDS-polyacrylamide gel and electroblotted onto a polyvinylidene fluoride membrane.
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