Sentence examples for volume of dna from inspiring English sources

Q: DNA copy determined from standard curve; VE: Volume of DNA eluent (50 μL); VA: Volume of DNA template amplified (five μL); VP: Volume of plasma used for DNA extraction (200 μL).

The solvent accessible area surface and volume of DNA were determined and significant differences observed under higher energy and temperature conditions.

This is due to the requirement that the volume of DNA specimens used in forensic DNA profiling must be at least 20 ng/ml (Notosoehardjo 1999).

At the final point, 50 μl of distilled water was added to re-suspend formed pellet then vortexed to make ready volume of DNA pellet for Polymerase Chain Reaction (PCR) and spectrophotometer.

PCR reactions were performed in 10 μL volumes containing 5 μL PerfeCTa™ SYBR green master mix (2×), 0.3 μL each primer (10 μM; 300 nM final concentration), 1 μL volume of DNA template (1 20 ng), and 3.4 μL of PCR-grade water (Solis BioDyne, Tartu, Estonia).

ASO-tsRNA co-transfections used ssDNA to control for total volume of DNA.

Reverse transcription was carried out using equal volume of DNA-free RNA from each fraction with the M-MLV reverse transcriptase and the oligo-dT primer (Invitrogen).

This means that even relatively small problems would require unmanageable volumes of DNA (on the order of large bathtubs) in order to represent all possible answers.

The University of Dundee team hope to help researchers make better use of the large volumes of DNA sequence data generated in modern bioscience.

Complete Genomics, which uses a technology that scales up to be cheap when large volumes of DNA are decoded at once, has set up what is essentially a sequencing factory that drug companies like Eli Lilly and Pfizer are using.

The detection threshold was one to two copies as assessed with serial dilution of gag containing plasmids in different volumes of DNA extracts obtained from PBMC or from microdissected tissues of uninfected macaques.