Sentence examples for volume of dilution from inspiring English sources

The extract was centrifuged twice (10000 g for 10 min at 4°C; 13000 g for 30 min at 4°C), the cleared supernatant diluted with 1 volume of dilution buffer [10 mM Tris/HCl (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 1 mM PMSF] and incubated with 25 μl of GFP trap beads (Chromotek) for 1.5 h at 4°C on a rocking shaker.

The lysate was separated into three samples diluted with an equal volume of dilution buffer [50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1%Triton X-100, 0.1%SDS, and a proteinase inhibitor cocktail (Complete Midi)].

The most effective dose, volume of dilution, number and sites of injection required for effective treatment are not well known [3].

The internal volume of dilution tunnel is 0.04 m, resulting in a residence time in the tunnel of 60 s.

Appropriate dilution of the culture was performed and the same volume of dilution was spotted onto 6 LB-N medium plates and onto 6 LB-N medium plates with Arbutin 90 mM being added.

Biological titer (BT = TU/ml, transducing units) was calculated according to the following formula: TU/μl = (P × N/100 × V) ×1/DF, where P = % GFP+ cells, N = number of cells at time of transduction, V = volume of dilution added to each well = 20 μl and DF = dilution factor.

After sonication, lysates were precleared for 1 h, diluted with nine volumes of dilution buffer (0.01% SDS, 1.2 mM EDTA, 16.7 mM Tris HCl (pH 8), 1.1% Triton X-100 and 167 mM NaCl) and incubated with the specific antibodies overnight.

Sonified chromatin was diluted with 9 volumes of dilution buffer (50 mM Tris HCl pH8, 5 mM EDTA pH8, 0.5% NP-40, 200 mM NaCl and 1 mM PMSF) and pre-cleared for 1 hour with Protein A Agarose/salmon sperm DNA (Millipore).

The supernatant fractions were pre-blocked with protein G sepharose beads in nine volumes of dilution buffer (lysis buffer) for 1 h at 4°C and centrifuged; subsequently, the supernatant fractions were incubated with 2 ug goat anti-AhR antibody overnight at 4°C with gentle rotation.

After 15 min, equal volume of 2× stop solution (20 mM Tris-HCl, pH 7.5, 2% Sarkosyl, 20 mM EDTA, 2× Complete mini) was added and incubated on ice for 15 min, then 7 M CsCl was added to a final concentration of 0.5 M. Before immunoprecipitation, 3 volumes of dilution buffer (10 mM Tris-HCl, pH 7.5, 10 mM EDTA, 100 mM NaCl, 1× Complete mini) were added.

(With higher substrate concentrations, we could avoid the sample handling problem by assaying the same volume of each dilution, but the same approach with more dilute substrate would yield samples with no counts).