Sentence examples for volume of blocking from inspiring English sources

The volume of blocking solution should be at least 20 ml per slide.

In short, standard ELISA assay plates (Corning) were incubated overnight with chemokine specific capture antibody, blocked with 1% (w/v) BSA in PBS for one hour and incubated with tissue homogenate (one volume homogenate diluted in one volume blocking solution for all chemokines except KC which was diluted in 4 volumes of blocking solution) for 2 hours.

Four volumes of blocking buffer (HEN buffer, 2,5% SDS, 25 mM methyl methanethiosulfonate, MMTS) were added to the supernatant (adjusted to 1 mg protein per ml) and maintained in shaking at 50 °C for 30 min.

Samples were diluted to 0.8 μg/μl in HEN buffer+0.04% CHAPS (C9426; Sigma-Aldrich) and incubated at 50°C for 1 h in 4 volumes of blocking solution: 9 volumes of HEN, 1 volume of 25% w/v SDS (L3771; Sigma-Aldrich) in ddH2O, and 20 m M S-methyl thiomethanesulfonate (64306; Sigma-Aldrich) from the 2 M stock solution in dimethylformamide.

Numerical examples show that uncertainties do affect the optimal solution and lead to an increase in volume of blocks compared to that from deterministic optimisation.

Add the cells for samples #2-5 in a volume of 10-50μl 10-50μlg blockingnto 96-well V-buffer plates.

Conditioned media were collected after 5 hrs of treatment and diluted with one volume of MSD blocking buffer (1% BSA in MSD wash buffer).

According to the above description of steam breakthrough, the calculation of blocking volume is divided into near-wellbore part and interwell part.

According to the judgment based on CIP and considering the design of blocking volume, all producers are divided into complete steam breakthrough wells and incomplete steam breakthrough wells.

A volume of 100 μl of blocking solution was then added to each well and gently mixed for 30 seconds.

So for some calculating methods of blocking agent volume, this kind of well is not taken into consideration.