Sentence examples for volume of binding from inspiring English sources

Therefore, sera from the three mice of each group were pooled and diluted with an equal volume of binding buffer (20 mM Na-phosphate, pH 7.0).

Cells were washed and incubated with varying concentrations (0.10 nM –500 nM final concentration) of biotin-labeled aptamer in a 200 µl volume of binding buffer containing 10% FBS.

This was followed by centrifugation (15 000 g, 30 min, 4°C) of the cell lysate, after which the supernatant was filtered and mixed with an equal volume of binding buffer (50 mM Na-carbonate buffer (pH 11) containing 1 M NaCl).

Reactions (2 µl) containing PPARα, PPARβ or PPARγ alone or together with RXRα were added to 9 µl total volume of binding buffer (10 mM Tris HCl, 50 mM NaCl, 0.5 mM dithiothreitol, 0.5 mM EDTA, 1 mM MgCl2, 4% glycerol, 0.5 mg/ml poly (dI-dC) at 25°C for 15 min followed by another 20 min incubation with 10 µCi of [γ-32P]ATP-labeled oligonucleotide containing the putative human PPRE binding sites.

Equal volume of binding buffer was gently mixed with the pooled PCR products and incubated for 5 min at room temperature.

Yeast lysates expressing mVps45 were grown to mid-log phase, pelleted, resuspended in 1/100th volume of binding buffer (40 mM HEPES pH7.4 KOH, 150 mM KCl, 1 mM DTT, 1 mM EDTA, 0.5% (v/v Triton X 100) then lysed by vortexing with 425-600 µ glass beads.

The column was prepared with 5 column volumes of binding buffer (20mM Tris-HCl pH 7.4).

Briefly, sera (0.4 mL) were diluted 1∶5 in binding buffer, passed 3 times over 2 mL of packed resin and washed with 15 column volumes of binding buffer.

Following a wash with at least 40 column volumes of binding buffer, bound ssDNA was centrifuged at 12,000 rpm for 5 min, and the supernatant was discarded.

An Ni-NTA column (5 ml) was set up in a cold room and equilibrated with 5 column volumes of binding buffer.

For IMAC of brain supernatant, cortex was homogenized in 30 volumes of binding buffer containing protease inhibitor then 500 µl of supernatant fraction was applied directly to the column.