Sentence examples for volume of ammonium from inspiring English sources

Then 0.3 volume of ammonium acetate and 0.7 volume of isopropanol was added and DNA was precipitated by gentle mixing and the mixture was centrifuged for 20 min.

The crystal phases purity and the mass of powder batch were the optimized responses of the powder synthesis and the concentration of calcium ions and volume of ammonium hydroxide were the experimental variables.

mRNAs were DNase treated and precipitated with 1 volume of ammonium acetate 5 M (2.5 M final concentration).

An equal volume of ammonium molybdate (1%) was added to the film and immediately drained with filter paper.

DTPA derivatives of f-MWNT (1 mg/ml in water) were diluted with an equal volume of ammonium acetate buffer (0.2 M, pH 5.5) yielding a final acetate buffer of 0.1 M, pH 5.5, to which InCl3 was added.

After centrifugation, supernatants were mixed with an equal volume of ammonium acetate (pH 4.0), and the mixtures from D1, D2 and D3 assays were analyzed by HPLC as described [29].

The fragments were then precipitated with 0.1 volumes of ammonium acetate 5 M and 2 volumes of absolute ethanol at -20°C.

Erythrocytes were lysed with 3 volumes of Ammonium Chloride (Stem Cell Technologies) and debris removed by filtering cell suspension through a 40 µM nylon cell strainer.

The samples were then diluted with 1.5 volumes of ammonium bicarbonate 100 mM and incubated with LysC endoproteinase (WAKO) for 7 hours at 37 °C; after LysC digestion the samples have been further diluted with 1.5 volumes of ammonium bicarbonate 50 mM and incubated with 10 μl of immobilized trypsin (Applied Biosystems) under rotation for 16 hours at 37 °C.

0.6 volumes of isopropanol and 0.4 volumes of ammonium acetate 10 M were added, gently mixed by inversion, incubated for 30 min on ice and centrifuged (15 min at 12,000 g).

Then, 25  μL of the bacterial suspensions was mixed with equal volumes of ammonium sulfate at various molarities (0.2 M to 4.5 M in 0.002 M PBS (pH 6.7)) in 96-well tissue culture plates.