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The reaction was stopped by adding an equal volume of acidic (2N HCl) stop solution.
Lysates were extracted using an equal volume of acidic phenol: chloroform: isoamyl alcohol (50: 50: 1) and then chloroform: isoamyl alcohol (49: 1).
The reaction was preincubated for 5 min at 30 °C prior to the addition of enzyme and quenched 5 min thereafter by adding one-fourth volume of acidic malachite green dye solution.
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Purification of this water may be a problem for future operations due to the economic and engineering difficulties associated with the treatment of large volumes of acidic, salty and organic-rich waters.
> In a 50-ml sterile tube, prepare a mix of collagen/1× DMEM by mixing 1 volume of 10× DMEM and 9 volumes of acidic collagen solution.
The aqueous phase of the suspension was extracted twice with equal volumes of acidic phenol/chloroform (Amresco), followed by an equal volume of chloroform.
Calculate the total amount of collagen gel to be used as a first layer for the experiment according to Table 22.> In a 50-ml sterile tube, prepare a mix of collagen/1× DMEM by mixing 1 volume of 10× DMEM and 9 volumes of acidic collagen solution.
Apparently, the longer evaporation time of the larger volume of the acidic solvent promoted the degradation as the radiotracer was exposed longer to the acidic milieu.
However, if a pH-dependent dissolution profile is necessary, the required volume of the acidic acceptor fluid is too large, since T20 does not increase CC solubility in acidic conditions so dramatically.
In order to meet the requirements for radiopharmaceutical applications, the obtained 47Sc eluate needed to be of high chemical purity and concentrated into a small volume of moderately acidic eluate to facilitate efficient radiolabeling and subsequent in vivo application.
These low intensities were registered even when methanol volume used for dissolution of the residue was four times smaller than initial volume of the acidic extracts.
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