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The skillet in question measures 3 inches deep and 20 inches across, giving it a heating surface of more than 2.2 square feet and a volume of more than 4 gallons: four times the area and eight times the volume of a standard 10-inch skillet.
Wall effects can therefore be ignored despite the small filtration cell diameter, allowing tests to be performed with only about one-tenth of the slurry volume of a standard commercial cell.
These standards are carefully added by pipetting the appropriate volume of a standard solution onto the sample matrix prior to extraction.
The projection patterns of VPM or POm axons measured in different cortices define the volume of a "standard projection column" characterized by its average cross-sectional area and length between pia and SCWM.
Extract twice with equal volume of a standard phenol/ chloroform mix and precipitate the water phase by adding 1/10 volume of 3 M NaOAc (pH 5.1) followed by 2 volumes of ethanol.
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For the calibration, four different volumes of a standard solution of nucleosides or creatinine were mixed with 0.5 ml of the internal standard (3-methyluridine), were treated separately on the SPE columns like the urine samples and were determined at 210, 245, 254 and 280 nm.
Typically, 50 μg lysate was labeled with 400 pmol Cy3 or Cy5, while the same volume of a pooled standard containing equal quantities of all samples was labeled with Cy2.
For example, for all patients, a mean loss of 597 mm3 of global cartilage volume with a standard deviation of 459 mm3 was detected at 24 months, a change that was smaller than the mean.
The reaction volume in the presented chip is 0.1% of the volume in a standard 96-well plate, meaning a 99.9% cost saving in reagent usage.
The measurement provides information on the nasal luminal anatomical structures, either as a measure of nasal volume over a standard distance into the nostril or as the minimal cross-sectional area within the nasal cavity.
The final standard was prepared by transferring an appropriate volume of primary standard into a 3-L Tedlar bag (SKC, USA) filled with 1 L of pure nitrogen.
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