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Time interval = 30 min. Volume masking of lateral line tissue and spot segmentation of nuclei and smFISH signal (membranes in green, nuclei in blue and pea3 transcripts in grey).
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The μPG 101 is an easy to use micro pattern generator for direct writing applications and low volume mask making.
The signal in the PGP stack was thresholded and used to create a volume mask for the bright PGP- positive fibers.
For each subject, the number of TT voxels was first counted up and then distributed at random within a brain volume that was defined as the intersection of all brain volume masks from individual subjects.
This might be due to the high respiratory rate in this group leading to a slight resistance to inspiration caused by inhaling from a small volume mask entraining a low flow of air [ 28].
The helically symmetrized volume was masked and used as the reference volume in the next iteration (Clemens et al., 2015).
They also hardly leak any sound at all, so you can raise your listening volume to mask out more sound without bothering those around you.
Very bright pixels from background noise were made into a volume and masked onto the original channel and removed.
MS lesions (previously coregistered to the b = 0 volume) were masked out from the DT-derived maps.
Small amount of viruses (2 × 10 PFU) was injected intratumourally, as otherwise the shrinkage in tumour volume would mask our attempt to correlate the transcriptional activities of the viruses to the proliferating tumour cells.
Using the empirical small-volume mask, a cluster size of 45 voxels (0.36 mL) was required.
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