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The band at the 23 25% interface was collected and diluted with the same volume isolation buffer.
We developed a modified protocol in compliance with the recommended biosafety guidelines from the World Health Organization based on the use of the MagNA Pure total nucleic acid large volume isolation kit for the extraction of SARS-coronavirus RNA.
According to the manufacturer's information, 2 kits, namely the MagNA Pure LC total nucleic acid isolation kit (Roche Diagnostics) and the MagNA Pure total nucleic acid large volume isolation kit (Roche Diagnostics), are recommended for use with the MagNA Pure LC instrument for the extraction of viral DNA or RNA from serum or plasma.
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The pellet was resuspended in an appropriate volume of isolation buffer, and centrifuged at 12,000×g for 8 min.
The pellet was resuspended in half of the original volume of isolation buffer and centrifuged in the way mentioned above.
The pellet was resuspended in an appropriate volume of isolation buffer without BSA and the protein concentration was determined according to [19].
Similarly it is not surprising that there should be a different relationship between volume and isolation rate for different bacteria.
The supernatants were collected in separate tubes and the pellets re-suspended in half a volume of isolation buffer each, and homogenized and centrifuged as before.
21 The mitochondrial pellet was washed twice and resuspended in a minimal volume of isolation medium and kept on ice until used.
Although these results are consistent with the direct measurement of islet volume after isolation from abruptly deceased healthy organ donors, the correlation between β-cell mass and BMI remains weak (r ≤ 0.5) (2, 3).
The suspension was diluted with an equal volume of isolation medium supplemented to 0.2% (wt/vol) with defatted BSA and homogenized in a Potter Elvehjem homogenizer with a loose-fitting Teflon pestle (10 strokes).
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