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The higher striatal volume in the template is due to individual variation, coregistration, and averaging.
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Polymerase chain reaction (PCR) of 16S rRNA gene was performed in 25 μL volume mixing the template DNA (10 ng) with 1x PCR buffer (Bioline), 2.5 mM MgCl2 (Bioline), 0.5 U Taq DNA polymerase (Bioline), 2.5 mM dNTPs each, 0.4 μM (each) primers fDl and rDl using a DNA thermal cycler (Applied Biosystems, USA).
For each subject, we obtained one parametric image of MRI-determined voxel-wise difference in volume, compared to the template brain.
The TDL was calculated from:
The 3D image volume at each time point was then realigned to this template by computing the combination of rotations (around the x, y, and z axes) and translations (in x, y, and z) that maximized the correlation between the image intensities of the volume in question and the template (rigid-body registration).
Photoluminescence spectra under ultraviolet region shows that the volume ratio of alcohol to water in the template has a great effect on the luminescence properties of ZnS Mn particles.
Operating costs (including costs of professional courses, information/ communication, licenses, and materials) were calculated based on volumes and unit prices as stated in the template.
Since all reverse transcription reactions were carried out in 20 µl reaction volumes, the template RNA amount ranged from 10, 100 and 1,000 copies per reaction (≈1 100 fg/µl).
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