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So, an alternative method which is suitable for up-scale PBR is necessary, while at the same time there must be consideration for practical light quantity in high volume cultivation.
However, in up-scale or large volume cultivation, mean light intensity is a better indication of light condition; light intensity at the illumination surface, IS and opposite surface, BS (end of light path) need to be considered (Ogbonna and Tanaka 2000).
Beginning 10 hours after induction, the culture volume was pumped through a tributylphosphate phase at a rate of 0.8% per minute (volume for extraction per volume cultivation medium).
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For scaling up the culture volume, the cultivation setup at pH 7, induction with 1 mM IPTG and with addition of 3% EtOH was used for the d-Hyd(co).
The integral volumetric specific productivity qSurfactin was determined using the maximal mass of Surfactin, the average medium volume and cultivation time (Eq. 6).
The corresponding enzyme concentrations (Cenz) and productivities (Prenz) per volume of cultivation medium were calculated using Equations (5) and (6), respectively.
As units, the yield per volume of cultivation broth (grams per 1,000 mL) and specific yield per biomass cell weight (grams per 1,000 mL) were measured at the end of cultivation.
For cellular dry weight, a known volume of cultivation broth was filtered using pre-weighted filters; these were washed with two volumes of distilled water and dried to constant weight at 105°C for 24 h.
The headspace H2 concentration was calculated by converting the H2 data (ml) obtained from GC to milli molar (mM) values using the ideal gas law equation (for room temperature) and volume of cultivation medium (l) [ 39].
E. coli (XL1-Blue-MRF') was cultivated in 2 L glass bottles up to a cultivation volume of 1.5 L.
A membrane-sparged helical tubular photobioreactor (MSTR) with a cultivation volume of 800 ml was designed in this study.
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