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Because most of the COPD patients are elderly [33], we decided to use a lung volume corresponding to a 65-year-old male.
Finally, a volume corresponding to a quantity of 1×106 cells was pipetted into a new culture flask, and culture media was added to a final volume of 20 mL.
Natural populations (1000 3000 m-2) of this species may pass a volume corresponding to a water column of 3 to 9 m through their burrows each day.[29] Consequently, their burrows are continuously flushed with overlying water resulting in highly oxic and oxidized conditions in burrow water and surrounding sediment (Fig. 7).
The protein eluted as a single peak (Fig. 4B) with a retention volume corresponding to a dimeric assembly (∼114 kDa).
BPL without its substrate eluted as a single peak at volume corresponding to a Mr of ∼29,500±150 Da, confirming that BPL exists mostly as a monomer in its native state.
The tumour volume corresponding to a 50% 5-year risk of metastases was calculated in each cohort.
Similar(49)
Therefore, discarding the lung volume corresponding to an airway pressure of 15 cmH2onon the ZEEP P V curve leads to an underestimate of lung derecruitment.
A volume corresponding to an estimated 1 µg of peptide was injected onto a 15 cm × 0.050 mm I.D. reversed phase column packed with 2 µm C18 beads (Acclaim PepMap RSLC analytical column, Thermo Scientific).
For the Western blotting, a volume corresponding to an OD260-value of 8 of each whole cell extract, or 0.1 μg recombinant protein, was electrophoresed in a 4-12% NuPAGE Bis-Tris gel (Invitrogen) under reducing conditions.
Second, using size exclusion chromatography, we found that MglA+ elutes as a monomer at an elution volume corresponding to an apparent molecular mass of 22 kDa irrespective of the nucleotide-bound state, whereas MglB (17 kDa) elutes at a volume suggesting that it is a dimer or tetramer.
It has been applied to large volumes corresponding to a room with a vibrating concrete wall and to a train with a vibrating window.
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