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Real Time PCR reactions were done in a 10 μl volume comprising of primer, cDNA template and 1× SYBR Green PCR master mix (Applied Biosystems).
PCR was done in 10μl reaction volume comprising of 05μl SYBR-Green qPCR master mix (KAPA Biosystems, USA), 10 pmol forward and reverse primers and 20ng genomic DNA.
The product of CO and mean transit time is the volume of distribution of the thermal indicator, or "intra-thoracic thermal volume" comprising of the intra-thoracic blood volume and the extravascular lung water.
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Figure 11 shows a reconstructed volume comprised of serial block-face images collected with 3-keV electron-probe energy and 1.5-keV landing energy.
In this proposed study, we will sample a large brain volume comprised of 95% white matter.
The q-PCR mixture (20 μl per volume) comprised of 10 μl SYBR® Premix Ex TaqTM (Takara, China), 0.4 μl of each primer (10 μM) (Table 3), 2 μl of cDNA, and 7.2 μl of RNase-free water.
Reactions were performed in a 25 μl volume comprised of 1 μl cDNA, 12.5 μl of 2× iQ SYBR Green Supermix (Bio-Rad) and 10 pmol of each primer.
As tertiary referral hospitals in the largest cities in the country, these two facilities receive large patient volume comprised of both initial patient presentations and district and regional referrals.
A slim, gorgeous-looking volume, comprising solely of The Metamorphosis and a thought-provoking introduction by David Cronenberg.
The remaining portions of the stand volumes comprised of Scots pine (Pinus sylvestris L). and various broadleaf species, mainly silver birch (Betula pendula Roth), downy birch (Betula pubescens Ehrh).
Reactions were performed in 25μL volumes comprised of 1× HiFi buffer, 2 mmol/L MgSO4, 0.2 mmol/L dNTPs, 0.4 μmol/L each primer, 1 μL template cDNA, and 1 U enzyme.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com