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So combined with field reality, several basic geology and production parameters are selected as basic parameters, such as permeability, porosity, net pay, injection temperature, production temperature, steam injection volume (cold water equivalent), liquid production rate and oil production rate.
"It's an honor that we welcome," said Chambers, who has lived at Pipe Creek Farm off and on since the age of 2. Maintaining it as a working farm is, he said, his way of paying tribute to his father's conversion to a simple life of manual labor, expressed in the volume "Cold Friday". "Here I determined to root the lives of my children...
The reaction was stopped by adding 10x volume cold PBS.
TNET- or SDS/TNET-solubilised, chemically cross-linked membrane proteins were diluted with an equal volume cold (4 °C) TNE (20 mM Tris HCl, pH 8.0, 130 mM NaCl, 5 mM EDTA, 1 mM PMSF) and loaded onto a small column (1 × 1 cm) of monoclonal anti-stomatin antibody GARP-50 covalently bound to CNBr-activated Sepharose (1 mg/ml), as described [1].
After incubation, 500 μl chloroform: isoamyl alcohol (24: 1) was added, mixed and then centrifuged at 13,000 rpm for 10 min. The top aqueous phase was removed to a new tube, added with 2/3 volume cold isopropanol, and mixed gently to precipitate DNA.
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Large-volume cold intravenous infusion of crystalloids has been used for induction of therapeutic hypothermia after cardiac arrest.
The technique of large-volume cold intravenous infusion has been found to be a simple, efficient and safe method of inducing therapeutic hypothermia (TH) in cardiac arrest survivors [ 1, 2], therefore, it has been widely adopted [ 1, 3].
In conclusion, large-volume cold intravenous infusion of normal saline resulted in more intense decrease in cerebral and pulmonary artery BT than colloid infusion of the same temperature and volume in this model of cardiac arrest.
Alternatively, membranes were dissolved in 1% SDS for 5 min at 37 °C, and the solution was diluted with 10 volumes cold (4 °C) TNET before immunoisolation.
Isolated DRMs were cross-linked with either 16 μM or 8 μM EGS, quenched with 15 mM Tris HCl, pH 8.0, dissolved in 1% SDS at 37 °C, and the solution was diluted with 10 volumes cold (4 °C) TNET before immunoisolation.
After sonification (10 min) and vortexing (10 min), the samples were subsequently deproteinised by adding 1 volume of cold sulphuric acid (0.33 M) and 1 volume of cold sodium hexametaphosphate (5 g/100 ml).
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