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Briefly, the microarrays were prehybridized, the Cy5- and Cy3-labeled cDNAs were mixed with hybridization buffer (30 µl final volume), applied to the microarray, and covered with a Hybri-slip HS (Sigma-Aldrich, Taufkirchen, Germany).
There was good reproducibility of volumetric measurements of whole-tumor and necrotic-tumor volume applied to tumors that had undergone radioembolization, with no significant difference between the first and second measurements within any of the three observers (p > 0.05).
A 6 mL/kg tidal volume applied to these 'baby lungs' results in airway pressures in the range of 30 to 35 cm H2O (which in many patients is likely to be injurious) [ 36].
The concentration (in milligrams per milliliter) of total IgG was calculated on the basis of the initial plasma or SF volume applied to the Protein G column and the amount of IgG eluted from the Protein G column (that is, before dialysis and filtration).
The result of this PCA based reconstruction of blood volume can be seen in Fig. 4. Figure 4a shows the PCA based reconstruction of blood volume applied to all 10 wavelength sets of the first image set (uniform thickness); Fig. 4b applied to the second image set (spatially varying thickness).
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Alternatively, increased perfusion rates and/or volumes applied to cisterna magna infusion did not appreciably alter the distribution of the tracers (data not shown).
Lower limits of quantification for the different volumes applied to the wells of the microtiter plate were calculated as follows: 1.22 nmol/well (100 μl), 0.91 nmol/well (200 μl) and 1.01 nmol/well (370 μl).
The registration between the FA template and the MNI152 volume was applied to the volume with the WM parcellations, and the resulting volume was used to extract the skeletonised DR data from each WM ROI.
The upper phase was transferred into a 50 ml disposable vessel, 1 volume of 80% ethanol was added and the total volume was applied to an RNeasy Maxi (Qiagen, cat. no. 75162) column.
This process is achieved with the use of a control volume methodology applied to each nodal element on a finite-volume mesh.
We analyze the stability of a reference finite volume scheme applied to this latter problem.
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