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As brain morphology research advances, it is critical to measure the reliability of these metrics using multiple volume acquisitions.
With time it was realised that QCT can also be performed on volume acquisitions of the spine.
The individual scans consisted of 60 whole brain volume acquisitions, divided into three cycles.
For each flow value, 10 volume acquisitions were done, reconstructed and averaged.
On each trial, a single syllable was played in the 800-ms silent interval between volume acquisitions (Fig. 1 D).
Between repeated orthogonal volume acquisitions, the device was reset and the subject re-aligned to the device.
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Producing these behaviors during volume acquisition introduces motion-induced signal changes that confound the activation signals of interest.
An alternative approach, that has only recently been applied to study single-word production, uses pauses in volume acquisition during the production of natural speech motion.
Using pauses or silent intervals in volume acquisition and block designs, results from individual subjects result in robust activation without motion-induced signal artefact.
We demonstrate that this large field-of-view, single-snapshot volume acquisition method -whichch requires only a simple and inexpensive modification to a standard fluorescence microscope -enableses scapture capture of coordinated activity patterns throughout mammalian neural volumes.
Sparse temporal acquisition schemes (STA) which use a single volume acquisition after each trial imply the advantage of auditory stimulation devoid of ambient scanner noise but have the drawback of a reduced statistical power.
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