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Stimulation waveforms were generated by an analog constant voltage stimulator (S48, Grass Instruments, West Warwick, RI) and a 10 Volt signal was split and sent two DS3 constant current isolated stimulators (Digitimer, Hertfordshire, England), where the amplitude of the stimulation was set in mA.
NCS assesses both motor and sensory function via a voltage stimulator applied to the skin over different points of the nerve to be tested.
First, regular, 8 Hz, pacing stimuli used 2-ms square-wave pulses whose amplitude was adjusted to twice the excitation threshold (DS2A isolated constant voltage stimulator; Digitimer, Welwyn Garden City, UK).
These sites were: calcaneous (HL); medial aspect of the transverse arch of the foot (M-M); proximal end of the fifth metatarsal (M-L); head of the first metatarsal (F-M); and, distal end of the fifth metatarsal (F-L .Stimulation was provided by a Digitimer Constant Current High Voltage Stimulator (Model DS7AH) with trains oF-L .Stimulationses at 300 Hz.
The S1 S2 interval was initially equal to the pacing interval, then reduced by 1 ms with each subsequent cycle until S1 S2 = 6 ms. Both the WT and RyR2S/S hearts were stimulated using square-wave stimuli of 2 ms duration and amplitudes of twice diastolic excitation threshold (Sabir et al. 2007) (DS2A isolated constant voltage stimulator; Digitimer, Welwyn Garden City, Herts., UK).
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Initially, two constant current high voltage stimulators (model DS7A, Digitimer, Welwyn, Garden City, Hertfordshire, UK) delivered ten single stimuli twitches with biphasic square pulses at widths of 300 μs.
A high-voltage stimulator (Digitimer DS7AH, Digitimer, Hertfordshire, England) was used to deliver a square-wave stimulus of 0.2 ms duration with a maximal voltage of 400 V.
Electrical stimulations (400 V, rectangular pulse of 0.2 ms) were delivered by a high-voltage stimulator (Digitimer DS7AH, Digitimer, Hertfordshire, England).
Electrical stimulations (400 V, rectangular pulse of 0.2 ms) were delivered by a high-voltage stimulator (Digitimer DS7AH, Digitimer, Hertfordshire, UK).
A high-voltage stimulator (Digitimer DS7AH, Digitimer, Hertfordshire, England) was used to deliver the doublet (square-wave stimuli of 0.2 ms duration) with a maximal voltage of 400 V.
P-R interval during atrial pacing was monitored directly on an oscilloscope triggered from a timing device (Digitimer 4030) which was also used to trigger the stimulators driving the pacing and stimulating electrodes (Digitimer DS2 constant voltage isolated stimulators).
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