Of the 58 volatiles tested by EAG, 19 evoked a significantly sex-biased response and in each case greater electrical activity was recorded in male antenna.
The feeding intensity had also a significant direct effect on the emission of the majority of black poplar volatiles tested: all monoterpenes, the sesquiterpenes nerolidol and (E -β-caryophyllene, all nitrogE -β-caryophyllenetilE -β-caryophyllenee all the GLV (Z)-3-hexenitrogenate.
The fact that a common trend for the four analytes with changing p is observed (see Figures 3 and 4, Figure S1 in the Supporting Information) suggests that the time range for these processes is relatively similar for these volatile organic test gases.
Seeds exposed to the other 14 volatile compounds tested had intermediate levels of germination efficiency and seedling formation (Figure 2).
Among the volatile fractions tested those from A. abrotanum containing davanone or silphiperfolane derivatives showed the highest antifungal activity.
The array was able to clearly discriminate the 13 volatile compounds tested based on their hydrophobicity and hydrophilicity molecules as well as the molecular weight.
All volatile exposure tests were done with Arabidopsis thaliana ecotype Columbia 7. Surface-sterilization of seeds and seedling formation studies were conducted as described previously with slight modifications (Hung et al. [2013]).
Volatiles were tested by head space GC/MS on a Thermo system (Focus GC & DSQII) using a standard validated procedure.
To evaluate this question, we designed a procedure to collect peptide-free urinary volatiles and tested these volatiles for their ability to mediate chemosensory discrimination of MHC-congenic mice differing in their MHC genotype.
