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All other volatile sampling and analysis methods were as previously described [ 15].
Directly after volatile sampling, we determined the fresh mass (FM) of each trapped leaf for further calculations.
Tholl et al. (2006) give a more detailed description of volatile sampling techniques (Tholl et al. 2006).
As an example, the SPME (Solid Phase Micro Extraction) technique was developed for uncomplicated and fast volatile sampling in a static headspace.
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An innovative encapsulation technique designed for the DSC high temperature measurements of the volatile samples is presented in this study.
Details of the geometry of the interior of the source were shaped to yield a small Cs+ spot size with virtually no halo, minimizing background for measurement of volatile samples.
Microtitre plates were sealed with an adhesive film to prevent the loss of any volatile samples and incubated for 24 hours at 37°C.
The plant volatile samples were analysed by a gas chromatograph-mass spectrometer (GC type 6890, MSD 5973: Hewlett Packard; Wilmington, DE, USA) as described in [ 61].
Volatile samples were analyzed within 4 days of volatile collections by a gas chromatograph (GC, Agilent 6890 N) connected to an HP5 column (30 m × 0.32 mm internal diameter × 0.25 μm film thickness) and an Agilent 5975 mass selective detector associated to a thermal desorption system (TDS2, Gerstel, Mühlheim Germany).
Analytical multidimensional gas chromatography (MDGC) and the excellent separation efficiency it achieves serve advanced characterization of complex volatile and semi-volatile samples, which is unlikely to be accomplished by single-dimensional chromatography.
Besides the capability of working with ionization sources in the atmosphere, there is also an in-vacuum plasma ionization source, so that both volatile and non-volatile samples could be analyzed.
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