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For volatile collection, S. latifolia plants from seeds collected in two populations (Leuk, Switzerland, n = 25; Ribes de Fraser, Spain, n = 4) were grown in a common garden setup in a green house.
Using an automated push-pull volatile collection system (Analytical Research Systems, Gainsville, FL, USA), we collected plant-produced volatile compounds from exposed S. altissima and Z. mays plants before and after herbivore damage.
Several volatile collection techniques were employed including dichloromethane solvent extraction, solid-phase microextraction (SPME) and thermal desorption of VOCs entrained on Tenax GR.
All were subjected to the volatile collection system.
After biopsy and volatile collection, melanoma samples were analyzed and confirmed by H&E staining.
The headspace volatile collection setup was prepared as previously described [19].
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For volatile collections, aliquots (20 ml) of fresh batches were transferred to 100-ml beakers.
The emergence rhythm of O. dissitus was monitored at the same time interval as volatile collections.
Before volatile collections, the JA-treated plants and the controls were transferred from their vials to individual glass tubes filled with 50 mL of tap water.
Volatile collections using active charcoal are not the cause for this previously reported variation between field and lab volatile collections.
They designed the study, wrote the manuscript and carried out volatile collections.
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