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The volatile analysis was done by the SPME-GC/MS technique.
The volatile analysis was performed using a gas chromatograph/mass spectrometer (GC/MS) equipped with an olfactory detector.
The optimized HS-SPME conditions were used for volatile analysis of leaf (Hinge et al. 2016) and mature grain (Mathure et al. 2011) samples.
Volatile analysis indicated an increase in 1-penten-3-ol in the n-3 fortified dairy-based beverage systems during storage.
In comparative volatile analysis of aromatic and non-aromatic rice cultivars no difference was recorded in presence of volatile compounds but only in the quantity (Widjaja et al. 1996).
Further samples were powdered using liquid nitrogen and distributed into 3 aliquots and stored at −80 °C, one used for volatile analysis, 2nd for RNA extraction and 3rd for other metabolite analysis.
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Other studies showed that in comparative volatiles analysis of mature grains of scented and non-scented rice not a single compound or set of compounds defined a particular aromatic rice cultivar but only the concentration (Jezussek et al. 2002; Zeng et al. 2009).
The materials used for volatiles analysis were bought from a local H. coronarium cut flowers farm.
For leaf volatiles analysis, raw data files were converted to netCDF format and processed using the R (http://www.r-project.org) packages XCMS (Tautenhahn et al., 2008) and CAMERA (http://www.bioconductor.org/biocLite.R) as described (Gaquerel et al., 2010).
All of these three workshops located in the Jindong district of Jinhua City, Zhejiang Province, their coordinates are 119° 84′E-119° 98′ E and 29° 28′ N-29° 46′ N. At the middle of aging, hams were taken as samples for DNA extraction and volatile compounds analysis.
Five naevi samples and three melanoma lesions big enough to obtain two parallel 3-mm samples were used for both histology (H&E staining) and volatile collection analysis.
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