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After 1 month, melanocytes were transfected with a p3XFLAG-CMV™-14 expression vector (Sigma Aldrich, S. Louis, Missouri, USA) carrying the FKBP51 gene, or a void vector as control, Metafectene (Biontex, Munich, Germany).
Low-copy vectors expressing RPN4* or RPN4 from the native RPN4 promoter or a void vector were transformed into the rpn4Δ LEU2 strainn.
Liver-specific transfection was accomplished by hydrodynamic overload with 100 µg plasmid (void vector pLPCX, pGFP, or a 1∶10 ration of pGFP∶pTRβ) dissolved in 2 ml isotonic NaCl.
Negative controls used comparable preparations harbouring a void vector.
P. pastoris containing a void vector was used as a negative control.
No corresponding protein is observed in samples prepared from bacteria carrying the control void vector.
Similar(53)
Using two-dimensional discrete element method (DEM) simulations, we discover a simple and strong linear correlation between contact normal- and void vector-based fabric tensors for granular materials composed of non-elongated particles.
The limited results show that the change of void vector-based fabric tensor appears to be affected by the corresponding components of the particle orientation-based fabric tensor and contact normal-based fabric tensor.
The two shRNA-transfected RARA-sh18 and RARA-sh19 clones express < 10% of the RARα protein levels in the parental (data not shown) and void vector-transfected Vect-C6 or Vect-C8 cells (Fig 10D).
While the shRNA constructs and void vectors do not alter the basal growth rate of SKBR3 cells (Fig 10E), RARα knockdown attenuates the anti-proliferative action of ATRA (Fig 10F).
The recombinant plasmid or the void pET3a vector (control) were introduced into E. coli BL21 (DE3) pLysE cells, and expression of soluble acyltransferase was first tested at standard induction temperature (37°C for 8 h).
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