Also, is the physiological concentration of DltC acceptor protein, which presumably takes over the role of CoA in vivo, high enough to be consistent with the proposed reaction cycle?
It remains to be determined whether under normal expression conditions the Rap1 concentration in vivo is enough to support the transition between binding modes, even though Rap1 is a highly abundant protein.
This suggests that the affinity of the recombinant UGT for cinnamic acid in vivo is high enough to ensure adequate chlorogenic acid synthesis.
Secondly, the amount of A22 normally used in vivo is high enough to make MreB precipitate, as it is a very small molecule that probably intercalates many proteins including MreB.
As a result, only three of them, wsc1Δ, elo2Δ and elo3Δ, showed a significant decrease in the β-1,3-GS activity (Fig. 6 and data not shown) compared to the wild type strain, but still maintaining an in vivo GS activity enough for cell viability.
However, there is enough in vivo evidence implicating acyl glucuronides, which, when backed up by in vivo circumstantial and documented in vitro evidence, supports the view that reactive acyl glucuronides may initiate toxicity/immune responses.
This concept may create new opportunities for delivery of conventional drugs that are not effective enough in vivo and/or display serious extrahepatic side-effects.
Similar regions in proteins are widespread enough in vivo.
The estimated increase of the stainless-steel coronary stent temperature by ΔT = 0.8°C during MRI in vivo is thus small enough to be considered harmless to the human body.
Nevertheless, the affinity in vivo may be high enough to ensure adequate synthesis of chlorogenic acid by the glycosylation pathway.
