Pollen that had been germinated in vitro was placed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, under low vacuum (18 psi Hg) for 5 h at room temperature.
Regenerated in vitro plantlets were placed horizontally on filter paper saturated with liquid half-strength MS medium and sprayed 19 h and 1 h before screening with 0.1 mM luciferin dissolved in MilliQ water.
For in vitro culture, embryos were placed in 20 μl drops of KSOM medium (Millipore) under mineral oil (Irvine Scientific) in an atmosphere of 5% CO2 in air, at 37°C, in groups of 10-15 per drop.
In vitro human blood clots were placed at the focus of a piezoelectric transducer.
To overcome the limitations of the in vitro culture system, explants were placed in a hypoxic chamber (0.5% O2) at the time of progesterone withdrawal to more accurately simulate the in vivo environment.
To evaluate the migration of TH17 cells, 5-µm Transwell inserts (Corning, Cambridge, MA) containing 1×105 in vitro-generated TH17 cells were placed in the 24-well plate so as to make contact with 600 µl of the medium alone (basal) or with 100 nM MIP-3α/CCL20 (R&D Systems, Minneapolis, MN).
For in vitro experiments, the optical fibers ends were placed inside the solution.
Sterile plants of both lines were placed from in vitro conditions to soil for adaptation, and then they were grown in greenhouse.
At 21 days in vitro, cortical neurons grown on MEAs were placed into the MED 64CH Integrated Amplifier interface and spike activity was recorded using Mobius software (both from AutoMate Scientific).
After recovering for up to 1 day in vitro, slices containing the corpus callosum were placed into the well of an open chamber fitted with a platinum electrode bottom (CUY700P10E, Nepagene).
