Sentence examples for vitro were obtained from inspiring English sources
Images of hBMSCs implanted in defects in vitro were obtained by a Spin Echo sequence (TR 1500 ms, TE 40 ms, FOV 40 mm, matrix 512×512, scanning time 14 min. 42 sec).
Alternatively, macrophages derived from bone marrow in vitro were obtained by isolating bone marrow cells from tibias and femurs, seeding the cells on 6-wells plates (3×105 cells/well) and culturing them in the presence of 10 ng/ml Macrophage Colony Stimulating Factor (Biosource International, Camarillo CA, USA) [27].
Cytokines and antibodies for T-cell differentiation in vitro were obtained from R&D Systems (Minneapolis, MN, USA).
Eggs of good quality that could be fertilized in vitro were obtained only after a substantial incremental increase of the amount of ovarian tissue transplanted.
The MC38/EGFP mouse colon cancer cells, transduced with green fluorescent protein gene and cultured in vitro, were obtained from the Institute of Immunology and Experimental Therapy, Wroclaw, Poland [ 25].
Fifty percent effective concentrations (EC50s) for small-molecule inhibition of parasite growth in vitro were obtained by quantification of red blood cell infection by fluorescence staining of DNA and fluorescence-activated cell sorting (FACS) analysis.
More importantly, the drug from the composite nanofibers can be released in a sustained and prolonged manner, and thereby, a significant antitumor efficacy in vitro is obtained.
Last, by using zebrafish embryo models, confirmation of the let-7a effects observed in vitro was obtained in vivo.
A maximal TEER value of 43.6 ± 3.89 Ωcm (after 3.19 days in vitro) was obtained when hBMEC cells were cultured under normal growth conditions (growth medium containing 20% FBS) at a seeding density of 4.5 × 10 cells/cm (Table 2).
γ-Secretase inhibitor I (in vitro) and GSI XX (in vitro, in vivo) were obtained from Calbiochem (San Diego, CA, USA).
Regardless of the cryoprotectant used, lower rates of in vitro development were obtained when the embryos were cryopreserved at the morula stage, and higher rates achieved using embryos at blastocyst stages.
